Fathers' time with children at the crossroads of the gender revolution: A comparative analysis in France, Italy, Sweden and the UK.

BACKGROUND According to recent literature the increasing women's labour market participation is only the first part of the so called gender revolution, while a second part is now unfolding, with an increased participation of men in family life with special attention to childcare. OBJECTIVE The aim of this paper is to explore fathers' involvement in parenting tasks within different contexts in terms of gender regimes, family policies, and workplace culture. The idea is to evidence individual factors that may enable/challenge the capability of fathers to stay with children and care for them, and to suggest opportune father-friendly policies. METHODS Time with children is compared among a sample of fathers in Time Use survey in France (2009-2010), Italy (2008-09), Sweden (2000-2001) and the UK (2000). Three different measures of father involvement are examined: the total time father spend with their children, the time they spend alone with them, and their engagement in childcare activities. RESULTS Results show that distinct micro-level factors contribute in determining the three levels of father's commitment analysed. Few cross-countries differences emerge. Fathers' involvement is mainly determined by their work-related features, by their children characteristics, and by their partner's working schedules. Weekday and weekend differences are observed. The quantum of father engagement strongly depends on the countries' institutional context: it is the highest in Sweden and the lowest in Italy. CONTRIBUTION This comparative study shows the methodological importance of considering different measures of father involvement to understand how micro-level factors influence the time fathers spend with their children in different institutional context

Active notch protects MAPK activated melanoma cell lines from MEK inhibitor cobimetinib

The crosstalk between Notch and MAPK pathway plays a role in MEK inhibitor resistance in BRAFV600E metastatic melanoma (MM) and promotes migration in GNAQQ209L uveal melanoma (UM) cells. We determined the cytotoxicity of combinatorial inhibition of MEK and Notch by cobimetinib and γ-secretase inhibitor (GSI) nirogacestat, in BRAFV600E and BRAF wt MM and GNAQQ209L UM cells displaying different Erk1/2 and Notch activation status, with the aim to elucidate the impact of Notch signaling in the response to MEK inhibitor. Overall the combination was synergic in BRAFV600E MM and GNAQQ209L UM cells and antagonistic in BRAF wt one. Focusing on UM cells, we found that cobimetinib resulted in G0/G1 phase arrest and apoptosis induction, whereas the combination with GSI increased treatment efficacy by inducing a senescent-like state of cells and by blocking migration towards liver cancer cells. Mechanistically, this was reflected in a strong reduction of cyclin D1, in the inactivation of retinoblastoma protein and in the increase of p27KIP1 expression levels. Of note, each drug alone prevented Notch signaling activation resulting in inhibition of c-jun(Ser63) and Hes-1 expression. The combination achieved the strongest inhibition on Notch signaling and on both c-jun(Ser63) and Erk1/2 activation level. In conclusion we unveiled a coordinate action of MAPK and Notch signaling in promoting proliferation of BRAFV600E MM and GNAQQ209L UM cells. Remarkably, the simultaneous inhibition of MEK and Notch signaling highlighted a role for the second pathway in protecting cells against senescence in GNAQQ209L UM cells treated with the MEK inhibitor

Synthesis, chemical characterization, and biological evaluation of a novel auranofin derivative as an anticancer agent

A novel gold(I) complex inspired by the known medicinal inorganic compounds auranofin and thimerosal, namely ethylthiosalicylate(triethylphosphine)gold(I) (AFETT hereafter), was synthesized and characterised and its structure was resolved through X-ray diffraction. The solution behavior of AFETT and its interactions with two biologically relevant proteins (i.e. human serum albumin and haemoglobin) and with a synthetic dodecapeptide reproducing the C-terminal portion of thioredoxin reductase were comparatively analyzed through 31P NMR and ESI-MS. Remarkable binding properties toward these biomolecules were disclosed. Moreover, the cytotoxic effects produced by AFETT on two ovarian cancer cell lines (A2780 and A2780 R) and one colorectal cancer cell line (HCT116) were analyzed and found to be strong and nearly superimposable to those of auranofin. Interestingly, for both compounds, the ability to induce downregulation of vimentin expression in A2780 R cells was evidenced. Despite its close similarity to auranofin, AFETT is reported to exhibit some peculiar and distinctive features such as a lower lipophilicity, an increased water solubility and a faster reactivity towards the selected target biomolecules. These differences might confer to AFETT significant pharmaceutical and therapeutic advantages over auranofin itself

Sex steroids, carcinogenesis, and cancer progression

The relationship between sex steroids and cancer has been studied for more than a century. Using an original intact cell analysis, we investigated sex steroid metabolism in a panel of human cancer cell lines, either hormone responsive or unresponsive, originating from human breast, endometrium, and prostate. We found that highly divergent patterns of steroid metabolism exist and that the catalytic preference (predominantly reductive or oxidative) is strictly associated with the steroid receptor status of cells. We explored intra-tissue concentrations and profiles of estrogens in a set of human breast tumors as compared to normal mammary tissues, also in relation to their estrogen receptor status. In particular, we showed that, with hydroxyestrogens representing the majority of all tissue estrogens, concentrations of individual metabolites, as well as their ratios, significantly differ when comparing normal tissue with cancer tissues or when they are related to the overall survival of cancer patients. © 2004 New York Academy of Sciences

Gly482Ser PGC-1α gene polymorphism and exercise-related oxidative stress in amyotrophic lateral sclerosis patients

The role of exercise in Amyotrophic lateral sclerosis (ALS) pathogenesis is controversial and unclear. Exercise induces a pleiotropic adaptive response in skeletal muscle, largely through the peroxisome proliferator-activated receptor γ coactivator 1α (PGC-1α), a transcriptional coactivator that regulates mitochondrial biogenesis and antioxidant defense mechanisms. It has been suggested that a Gly482Ser substitution in PGC-1α has functional relevance in human disorders and in athletic performance. To test this hypothesis, we examined the genotype distribution of PGC-1α Gly482Ser (1444 G > A) in ALS patients to evaluate whether or not the minor serine-encoding allele 482Ser is involved in oxidative stress responses during physical exercise. We genotyped 197 sporadic ALS patients and 197 healthy controls in order to detect differences in allelic frequencies and genotype distribution between the two groups. A total of 74 ALS patients and 65 controls were then comparatively assessed for plasmatic levels of the oxidative stress biomarkers, advanced oxidation protein products, ferric reducing ability and thiol groups. In addition a subgroup of 35 ALS patients were also assessed for total SOD and catalase plasmatic activity. Finally in 28 ALS patients we evaluated the plasmatic curve of the oxidative stress biomarkers and lactate during an incremental exercise test. No significant differences were observed in the genotype distribution and allelic frequency in ALS patients compared to the controls. We found significant increased advanced oxidation protein products (p A SNP, ALS patients with Gly482Ser allelic variant show increased exercise-related oxidative stress. This thus highlights the possible role of this antioxidant defense transcriptional coactivator in ALS

Influence of socio-demographic features and apolipoprotein E epsilon 4 expression on the prevalence of dementia and cognitive impairment in a population of 70-74-year olds: The InveCe.Ab study

Abstract The age-specific prevalence rates of dementia vary widely. Studies focusing on specific age groups are needed to provide reliable estimates for healthcare providers and policy makers. We estimated the prevalence of dementia, dementia subtypes and cognitive impairment in "InveCe.Ab" (ClinicalTrials.gov, NCT01345110 ), a single-step multidimensional population-based study of 70–74-year olds living in Abbiategrasso (Milan, Italy). We also looked for associations with socio-demographic factors and the presence of the apolipoprotein E-ɛ4 allele. The overall dementia prevalence was 3% (95%CI: 2.1–4.1%) [Alzheimer's disease (AD): 1.2% (95%CI 0.6–1.9%); vascular dementia (VD): 1.4% (95%CI: 0.8–2.2%)]. Being single was found to be a risk factor for vascular dementia; subjects born in southern Italy were shown to be at greater risk both of overall dementia and of vascular dementia. The prevalence of cognitive impairment, with or without subjective cognitive complaints (cognitive impairment, no dementia, CIND) was 7.8% (95%CI: 6.4–9.4%). As regards the CIND subgroups, the prevalence of subjects with subjective cognitive complaints (mild cognitive impairment, MCI) was 5.0% (95%CI 3.9–6.3%), while the prevalence of those without MCI (CIND-other) was 2.8% (95%CI: 1.9–3.8). The males had a higher risk of MCI and CIND-other; the older subjects were more likely to have MCI, and those born in north-eastern Italy to have CIND-other. The prevalence of AD was higher among the apolipoprotein E-ɛ4 carriers. Our data highlight the importance of dementia and cognitive impairment in the transitional period from adulthood to old age, and reveal the presence of different associations with socio-demographic and genetic factors

Linee guida per il Bilancio di Genere negli Atenei italiani

l Bilancio di Genere è un documento che, da un lato, fotografa la distribuzione di genere delle diverse componenti all’interno dell’Università nonché la partecipazione di donne e uomini negli organi di gestione dell’Ateneo e, dall’altro, monitora le azioni dell’Ateneo a favore dell’eguaglianza di genere, e valuta l’impatto di queste e delle politiche dell’Ateneo, compresi gli impegni economici-finanziari, su donne e uomini. Il Bilancio di Genere è dunque uno strumento essenziale per realizzare l’eguaglianza di genere nelle Università e per integrare la prospettiva di genere in tutte le politiche dell’Ateneo. L’importanza di portare la questione di genere al centro dell’attenzione degli Atenei, è evidente se si considera che in Italia, le donne rappresentano solo il 20 % dei professori ordinari e, tra i Rettori italiani, solo il 7% sono donne. Le forbici che descrivono le carriere di donne e uomini all’interno degli Atenei provano inoltre il c.d. fenomeno del “tubo che perde”: al progredire della carriera universitaria, il numero di donne diminuisce e l’Università perde le relative risorse. La diseguaglianza di genere causa dunque un problema di perdita di capacità e cattivo utilizzo di risorse pubbliche. La CRUI ha da tempo intrapreso un percorso a favore della tutela dell’eguaglianza tra donne e uomini nelle Università e ha assunto un impegno preciso per implementare e monitorare la diffusione e l’utilizzo del Bilancio di Genere quale strumento fondamentale per inserire la parità di genere nella più ampia strategia di sviluppo degli Atenei. A tal fine, ha dato mandato a un Gruppo di lavoro di esperte di elaborare le linee guida e la metodologia per realizzare il Bilancio di Genere delle Università e facilitare, così, una sua capillare diffusione tra gli Atenei italiani. Il volume illustrata nel dettaglio il processo di redazione del Bilancio di Genere - dalla costituzione del gruppo di lavoro, sino alla diffusione degli esiti del Bilancio di Genere - qual è il suo contenuto - mediante la individuazione di ambiti e fenomeni da analizzare con l’identificazione dei corrispondenti indicatori e delle relative rappresentazioni - e come il Bilancio di Genere debba essere adoperato come strumento di governance dell’Ateneo, attraverso l’integrazione con i principali documenti di programmazione e rendicontazione, al fine di promuovere l’eguaglianza sostanziale all’interno degli Atenei

Synthesis and Biological Evaluation of 2-Heteroarylthioalkanoic Acid Analogues of Clofibric Acid as Peroxisome Proliferator-Activated Receptor alpha Agonists

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The VEI 2 Christmas 2018 Etna Eruption: A Small But Intense Eruptive Event or the Starting Phase of a Larger One?

The Etna flank eruption that started on 24 December 2018 lasted a few days and involved the opening of an eruptive fissure, accompanied by a seismic swarm and shallow earthquakes, significant SO2 flux release, and by large and widespread ground deformation, especially on the eastern flank of the volcano. Lava fountains and ash plumes from the uppermost eruptive fissure accompanied the opening stage, causing disruption to Catania International Airport, and were followed by a quiet lava effusion within the barren Valle del Bove depression until 27 December. This was the first flank eruption to occur at Etna in the last decade, during which eruptive activity was confined to the summit craters and resulted in lava fountains and lava flow output from the crater rims. In this paper, we used ground and satellite remote sensing techniques to describe the sequence of events, quantify the erupted volumes of lava, gas, and tephra, and assess volcanic hazards.Publishedid 9056V. Pericolosità vulcanica e contributi alla stima del rischioJCR Journa